Anti-inflammatory effect of alcoholic Datura steramonium seed extract in acute inflammation induced by formalin injection in hind paws of male NMRI rats

In the present study, the effect of Datura steramonium (DS) seed extract on acute inflammation induced by formalin injection was investigated. For this purpose, two control and treatment groups were selected and in order to induce pain, formalin (50 µl, 2.5%) was applied to the plantar surface of hind paws. In treatment group, 20-30 min before formalin injection, the DS seed extract was used i.p. at an ED50 of 50 mg/kg. At the same time, Evans-blue dye (30 mg/kg) was injected via left ventricle. One hour after Evans-blue injection, the rat’s paw was detached and after crushing, it was mixed with aceton + sulfate sodium solution. The mixture set aside 24 h at room temperature, and then, the solution was centrifuged. Absorption of the solution was measured by spectrophotometer. Our results from control and treatment groups were analyzed by Student’s t-test. The results showed that there was a significant (P